Ribavirin uptake by human erythrocytes and the involvement of nitrobenzylthioinosine-sensitive (es )-nucleoside transporters
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چکیده
منابع مشابه
Species differences in nucleoside transport. A study of uridine transport and nitrobenzylthioinosine binding by mammalian erythrocytes.
A kinetic study of the inward transport of uridine in erythrocytes of rabbit, human, mouse, rat and guinea-pig demonstrated that the apparent Km of this process was similar (about 0.2mM) in these cell types, but Vmax. values differed markedly. In this array of cell types, Vmax. values were proportional to the number of transport-inhibitory, high-affinity binding sites present per cell of each t...
متن کاملMODULATION OF MITOCHONDRIAL UPTAKE OF A DENOSINE BY NITROBENZYLTHIOINOSINE
In this study the uptake and metabolism of adenosine by mitochondria has been investigated. Incubation of CEM cells mitochondria preparation with [3H] -adenosine showed substantial uptake and metabolism of adenosine. Adenosine was both anabolized to AMP, ADP and ATP, and also catabolized to inosine. The highest concentration of metabolites in extracted mitochondria was due to AMP. The mito...
متن کاملExtraction and partial purification of the nucleoside-transport system from human erythrocytes based on the assay of nitrobenzylthioinosine-binding activity.
Nitrobenzylthioinosine, a potent nucleoside-transport inhibitor, binds to high-affinity sites on the human erythrocyte membrane. This binding is a specific interaction with functional nucleoside-transport sites. The protein(s) responsible for high-affinity nitrobenzylthioinosine binding was purified 13-fold by treatment of haemoglobin-free 'ghosts' with EDTA (pH 11.2) to remove extrinsic protei...
متن کاملExpression of high levels of nitrobenzylthioinosine-sensitive nucleoside transport in cultured human choriocarcinoma (BeWo) cells.
We have examined binding of [3H]nitrobenzylthioinosine (NBMPR) and influx of [3H]thymidine in adherent cultures of human choriocarcinoma (BeWo) cells and, for comparison, cervical-carcinoma (HeLa) cells. Specific association of NBMPR with BeWo cells at 22 degrees C required 1.5 h to reach an equilibrium between free and bound ligand, whereas association with HeLa cells required 20-30 min. Scatc...
متن کاملMammalian nitrobenzylthioinosine-sensitive nucleoside transport proteins. Immunological evidence that transporters differing in size and inhibitor specificity share sequence homology.
Polyclonal antibodies were raised against the nitrobenzylthioinosine (NBMPR)-sensitive nucleoside transporter of human erythrocyte membranes. On Western blots of these membranes they labeled the broad "band 4.5" region (average apparent M(r) 55,000), which contains both the nucleoside and glucose transport proteins. However, they did not recognize the glucose transporter when this was prepared ...
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ژورنال
عنوان ژورنال: British Journal of Pharmacology
سال: 1998
ISSN: 0007-1188
DOI: 10.1038/sj.bjp.0701775